| Actor | Character | Firearm | Notes |
|---|---|---|---|
| Basil Rathbone | Sherlock Holmes | Colt 1878 Double Action | - |
| Basil Rathbone | Sherlock Holmes | Smith & Wesson New Model No.3 | Only on promotional images |
| E.E. Clive | Inspector Bristol | Colt 1878 Double Action | - |
| George Zucco | Professor Moriarty | Colt 1878 Double Action | Standard with 5" barrel, standard with 7.5" barrel and 1878-1902 Philippine Model |