| Actor | Character | Firearm | Notes |
|---|---|---|---|
| Basil Rathbone | Sherlock Holmes | Colt Police Positive | - |
| Don Terry | Howe | Colt Detective Special | - |
| Edmund MacDonald | Lt. Grogan | Colt Police Positive | - |
| George Zucco | Heinrich Hinkel alias Richard Stanley | Colt Police Positive | - |
| Henry Daniell | William Easter | Colt Police Positive | - |
| Nigel Bruce | Dr. Watson | Webley Bulldog | - |